Pierce peptide desalting spin columns. Each spin column can Introduction The Thermo ScientificTM PierceTM C18 Spin Tips enable fast and efficient capture, concentration, desalting and elution of peptides. Peptides are bound to the hydrophobic resin under acidic aqueous conditions and desalted by washing the column with acidified water by low-speed centrifugation. Pierce 1. Features of Screw Cap Spin Columns: • Adaptable: included Luer-Lok adaptors allow these columns to be used for syringe-based purifications • Column volume: 900μL • Resin volume: 20 to 400μL • Filter type: polyethylene Detergent Removal Spin Columns & Plates. These C-18 Spin Columns are ready-to-use centrifuge columns of porous C18 reverse-phase resin with excellent binding and recovery characteristics for peptide sample preparation for mass spectrometry and other methods. Pierce Peptide Desalting Spin Columns - New from Thermo Fisher Scientific - Supplies & Plastic Thermo Scientific Pierce Peptide Desalting Spin Columns are ready-to-use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. The polymer-based hydrophobic resin Peptide desalting spin columns are laboratory equipment used to remove salts, buffers, and other small molecules from peptide samples. Each spin column can Pierce C18 Spin Tips Easy-to-use C18 spin tips for fast and efficient clean-up of peptides for MS analysis Thermo ScientificTM PierceTM C18 Spin Tips enable fast and efficient capture, concentration, desalting, and elution of up to 10 μg peptides per 20 μL sample for MS analysis. Place in a 2mL tube and spin column at 5000 x g for 000100. Get tips and helpful information when starting with your protein dialysis, desalting, or concentration experiment from choosing the right method to help with setup to get optimal results. The spin column format allows simultaneous processing of multiple samples without the Thermo Scientific Pierce Peptide Desalting Spin Columns are ready-to-use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. Pierce™ Peptide Desalting Spin Columns. Each spin column can The kit includes sufficient material to process 10 samples of 250 µl each for most dyes. Each tip contains a C18 reversed-phase sorbent that minimizes flow resistance and provides excellent binding and recovery characteristics at a wide range of peptide concentrations. Available in 50カラム Pierce C18 Spin Tips Easy-to-use C18 spin tips for fast and efficient clean-up of peptides for MS analysis Thermo ScientificTM PierceTM C18 Spin Tips enable fast and efficient capture, concentration, desalting, and elution of up to 10 μg peptides per 20 μL sample for MS analysis. In addition, ensure that LC-MS pre-blended solvents are being used (formic acid/water, formic acid/acetonitrile, etc. Each spin column can Pierce Peptide Desalting Spin Columns provide excellent binding and recovery characteristics for native and TMT-labeled peptide samples in preparation for MS. Proteolytic digests of proteins extracted from cells or tissues are loaded onto an equilibrated desalting spin column. 89851, 89852), Pierce C18 Spin Tips (Cat. The kit contains 5 mL of Dye Removal Resin, 10 Spin Columns and 20 Collection Tubes. 5 mg/mL. Each spin column can View and Download Thermo Scientific 89852 user manual online. Separate proteins and other macromolecules from low-molecular weight buffer salts and reagents with Thermo Scientific Pierce 7K MWCO (molecular-weight cutoff) Polyacrylamide Spin Desalting Columns, which are porous polyacrylamide-based gel-filtration columns for biological sample preparation. The Thermo Scientific ™ Pierce ™ Peptide Desalting Spin Columns are optimized for desalting 5 µg to 5 mg of peptide sample from protein digests. Thermo Scientific Pierce C18 Spin Columns (10–150 μL) and Spin Tips (10–100 μL) effectively remove interfering contaminants, while also maintaining excellent binding and recovery of peptide samples for use upstream of mass spectrometry and other high sensitivity detection methods. Compatible—resin is validated with a variety of complex samples, including TMT-labeled peptides, and is stable under extreme pH conditions Pierce Peptide Desalting Spin Columns provide a convenient and reproducible way to desalt and remove contaminants from peptide samples prior to analysis by mass spectrometry. Also for: 89851, Pierce series. Thermo Scientific™ Pierce™ peptide desalting spin columns are ready to use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. Each spin column contains a porous C18 reversed-phase resin with excellent binding and recovery characteristics at a wide range of peptide concentrations. Desalting and buffer exchange are two of the most widely used applications in resin filtration chromatography. 89852 laboratory equipment pdf manual download. Available in 50 columns Thermo Scientific Pierce Peptide Desalting Spin Columns are ready-to-use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. Each 20μL tip can bind up to 10μg of total peptide and is ideal for Achieve excellent recovery of proteins with Thermo Scientific Pierce 7K MWCO (molecular-weight cutoff) Polyacrylamide Spin Desalting Columns, which are ready-to-use, disposable, gel-filtration columns. With Pierce Peptide Desalting Spin Columns, more than 90% of salt or labeling tags such as TMT tags can be removed. Each spin column can The Thermo ScientificTM PierceTM Peptide Desalting Spin Columns are optimized for desalting 5 μg to 5 mg of peptide sample from protein digests. Chromatography Thermo Scientific Pierce Peptide Desalting Spin Columns are ready-to-use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. ) Find additional tips, troubleshooting help, and resources within our Mass Pierce Peptide Desalting Spin Columns provide a convenient and reproducible way to desalt and remove contaminants from peptide samples prior to analysis by mass spectrometry. Thermo Scientific Zeba Spin Desalting Columns are made of low protein binding polypropylene and compatible with a wide range of standard laboratory instruments and consumables. Thermo Scientific Pierce Screw Cap Spin Columns are convenient tools for manipulating small volumes of affinity supports (20 to 400 μL) for protein purification. Thermo Scientific Pierce Peptide Desalting Spin Columns are ready-to-use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. 84850) or Pierce™ Peptide Desalting Spin Columns. Pierce™ Micro-Spin Columns. 1% triethylamine) and 12 spin columns containing pH-resistant, reversed-phase resin. Pierce™ peptide desalting spin columns provide a convenient and reproducible way to desalt and remove contaminants from peptide samples prior to analysis by mass spectrometry. Remove salts and small molecules from proteins and nucleic acids. Multiple samples can be processed in less than 5 Separate proteins and other macromolecules from low-molecular weight buffer salts and reagents with Thermo Scientific Pierce 7K MWCO (molecular-weight cutoff) Polyacrylamide Spin Desalting Columns, which are porous polyacrylamide-based gel-filtration columns for biological sample preparation. Nos. Each spin column can Thermo Scientific Pierce Peptide Desalting Spin Columns are ready-to-use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. The procedure is simple and requires less than 20 minutes to process protein digests, strong cation exchange fractions, and enriched phosphopeptides for mass spectrometric (MS) analyses. Protein Desalting Spin Columns are designed to desalt or exchange buffer of protein samples with volumes of 30-120 l. Pierce Peptide Desalting Spin Columns provide a convenient and reproducible way to desalt and remove contaminants from peptide samples prior to analysis by mass spectrometry. Various formats, MWCOs, and resins available, including spin columns, cartridges, and filter plates. Ready-to-use, gel-filtration Pierce Dextran Desalting Columns can separate proteins and macromolecules from low-molecular weight buffer salts or reagents up to 5000 Daltons (5 kDa) in 5- or 10-mL volu. Spin at 5000 x g for 000100and discard flow-through. Thermo Scientific Pierce Peptide Desalting Spin Columns are ready-to-use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. 1 Take a Pierce peptide desalting spin column and remove the white tip (do not remove the screw cap of the tube). Thermo Scientific Pierce Polyacrylamide Spin Desalting Columns, 7K MWCO (molecular-weight cutoff), are ready-to-use, disposable, gel-filtration columns for the separation of proteins and other macromolecules from low-molecular-weight buffer salts and reagents. Peptide Desalting Spin Columns. Each spin column can Pierce Peptide Desalting Spin Columns provide a convenient and reproducible way to desalt and remove contaminants from peptide samples prior to analysis by mass spectrometry. Each spin column can Product description Peptide samples can be purified and concentrated for a variety of applications using the Thermo ScientificTM PierceTM C18 Spin Columns. 89851, 89852) or Pierce C18 Resin. The columns effectively remove excess unreactive TMT label along with contaminating salts. Browse our catalog of desalting columns for size-exclusion chromatography. We recommend visual inspection of samples for particulate matter and using Pierce Peptide Desalting Spin Columns Thermo Scientific Pierce C18 Spin Columns (10–150 μL) and Spin Tips (10–100 μL) effectively remove interfering contaminants, while also maintaining excellent binding and recovery of peptide samples for use upstream of mass spectrometry and other high sensitivity detection methods. 2 Add 300 μLof acetonitrile. The Thermo Scientific Pierce Graphite Spin Columns enable fast and efficient capture, concentration, desalting and elution of hydrophilic peptides. Each spin column can The product 89851: Pierce™ Peptide Desalting Spin Columns, 50 Columns is not orderable at the moment. Each spin column can Thermo Scientific Pierce Polyacrylamide Spin Desalting Columns, 7K MWCO (molecular-weight cutoff), are ready-to-use, disposable, gel-filtration columns for the separation of proteins and other macromolecules from low-molecular-weight buffer salts and reagents. 8K MWCO Polyacrylamide Desalting Columns are pre-packed with porous polyacrylamide beads and provide for buffer-exchange and desalting of proteins Read more Thermo Scientific Pierce Peptide Desalting Spin Columns are ready-to-use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. These devices have exceptional desalting characteristics with 95% retention of salts and small molecules while providing excellent recovery of proteins greater than 7,000 Da. We recommend performing additional cleanup after protein digestion to remove any residual salts or partially digested proteins using Pierce Peptide Desalting Spin Columns (Cat. Each spin column can Description Thermo Scientific Pierce C18 Spin Columns (10–150 μL) and Spin Tips (10–100 μL) effectively remove interfering contaminants, while also maintaining excellent binding and recovery of peptide samples for use upstream of mass spectrometry and other high sensitivity detection methods. Pierce Peptide Desalting Spin Columns provide excellent binding and recovery characteristics for native and TMT-labeled peptide samples in preparation for MS. Each spin column can bind from 5 µg to 5 mg of native and TMT-labeled peptides. The polymer-based hydrophobic resin Thermo Scientific™ Pierce™ Peptide Desalting Spin Columns, 25 Columns PROMO Pierce Peptide Desalting Spin Columns provide a convenient and reproducible way to desalt and remove contaminants from peptide samples prior to analysis by mass spectrometry. 1. The kit includes a high-pH solution (0. For Research Use Only. Detergents or surfactants are important for solubilizing, stabilizing, and disaggregating protein complexes; however, detergents interfere with downstream analysis including ELISA, isoelectric focusing, and mass Pierce Peptide Desalting Spin Columns provide a convenient and reproducible way to desalt and remove contaminants from peptide samples prior to analysis by mass spectrometry. The ideal labeled-protein concentration range is 1-2. Pierce C-18 Spin Columns remove interfering contaminants and release peptides in MS-compatible solutions, providing an effective tool that aids in the MS examination of post-translational modifications or for the identification of proteins by peptide mapping. The polymer-based hydrophobic resin Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges. Available in 25 x 500 μL Columns Thermo Scientific Pierce Peptide Desalting Spin Columns are ready-to-use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. Each reversed-phase fractionation spin column enables fractionation of 10-100μg of peptide sample using a microcentrifuge. . The Thermo Scientific™Pierce™Detergent Removal Resin enables removal of commonly used ionic, nonionic, and zwitterionic detergents from protein/peptide digest solutions. Features of Micro-Spin Columns: Small volume. Each spin column can Description Pierce Peptide Desalting Spin Columns provide a convenient and reproducible way to desalt and remove contaminants from peptide samples prior to analysis by mass spectrometry. How do I determine if my sample is compatible and ready for LC-MS? Samples prepared using LC/MS grade reagents are suitable for LC-MS; however, particulates and other small molecules can all interfere with liquid chromatography separation and mass spectrometer source ionization. They operate on the principle of size-exclusion chromatography, allowing the larger peptide molecules to pass through while retaining the smaller contaminants. Each spin column can Pierce C-18 Spin Columns remove interfering contaminants and release peptides in MS-compatible solutions, providing an effective tool that aids in the MS examination of post-translational modifications or for the identification of proteins by peptide mapping. No. Pierce Peptide Desalting Spin Columns provide a convenient and reproducible way to desalt and remove MS interfering contaminants from peptide samples. Not for use in diagnostic procedures. Clean your MS system and/or perform sample clean-up using Pierce Peptide Desalting Spin Columns (Cat. Please contact our Technical Support for possible alternatives. Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center. The polymer-based hydrophobic resin contained in the column is stable from pH 1-14 and binds peptide samples with excellent recovery (typical recovery is >90%) under reversed phase conditions. Column Preparation 1 Column Preparation 1. The polymer-based hydrophobic resin contained in each column provides excellent binding and recovery characteristics for peptide samples in preparation for mass spectrometry and other methods. Thermo Scientific Pierce Micro-Spin Columns are convenient tools for manipulating small volumes of affinity supports (5 to 100 L) for protein purification. Dec 31, 2024 · Pierce Peptide Desalting Spin Columns provide a convenient and reproducible way to desalt and remove contaminants from peptide samples prior to analysis by mass spectrometry. Each spin column can If I already removed small contaminants and detergents at the protein level, do I still need to desalt my peptides prior to mass spectrometry analysis? Yes. Each spin column can Jan 30, 2015 · Pierce™ Dextran Desalting Columns (5K MWCO). Bind remove high detergent concentrations from samples with minimal sample loss using HiPPR and Pierce detergent removal resins in flexible formats. Multiple samples can be processed in less than 5 Pierce Peptide Desalting Spin Columns provide a convenient and reproducible way to desalt and remove contaminants from peptide samples prior to analysis by mass spectrometry. The polymer-based hydrophobic resin Thermo Scientific Pierce Peptide Desalting Spin Columns are ready-to-use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. Each spin column can Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges. Each spin column can The peak most likely represents PEG or a similar contaminant. juybngaxqqknrstsdpgu3apabyoytxymsf0i8